In Vitro Cell Viability Analysis Service (XTT-Based)

At SYNPHARM, in vitro cell viability/cytotoxicity analyses are performed using various cancer and healthy control cell lines in accordance with internationally accepted scientific standards. The studies are conducted using the XTT cell viability assay, which is based on the evaluation of cellular metabolic activity. The effects of tested compounds, extracts, nanoparticles, biomaterials, and formulations on cell proliferation and viability are analyzed in a reliable and reproducible manner.

During the analysis process, appropriate cell culture conditions are maintained, and parameters including cell density, incubation time, solvent controls, negative/positive controls, and absorbance measurements are optimized according to standardized protocols. Based on the obtained data, cell viability percentages, cytotoxic activity levels, and when required, IC₅₀ values can be calculated.

All analyses are conducted with a minimum of 3 independent replicates to ensure scientific reliability. Depending on project requirements:

• 24 hours (100 Euro / plate)
• 48 hours (125 Euro / plate)
• 72 hours (150 Euro / plate)

incubation periods can be applied.

Experimental design is optimized according to:

• number of cell lines used,
• number of applied concentrations,
• incubation duration,
• number of microplates,
• number of replicates,
• and requested additional analyses.

Available Cell Lines

At SYNPHARM, studies can be performed using cancer and healthy control cell lines representing different organs, tissues, and disease models. The following cell line panel can be utilized for anticancer activity screening, selective cytotoxicity studies, biocompatibility analyses, neurotoxicity investigations, oxidative stress studies, inflammation-related assays, vascular toxicity evaluations, wound healing studies, and biomaterial safety assessments.

CANCER CELL LINES

AGS – Human Gastric Cancer Cell Line

AGS is a human gastric adenocarcinoma-derived adherent epithelial cell line. It is widely used in gastric cancer biology, gastric epithelial damage, oxidative stress, inflammation, and anticancer agent screening studies. AGS serves as an important model for cytotoxicity and proliferation analyses targeting gastric cancer.

HCT116 – Human Colorectal Cancer Cell Line

HCT116 is a human colorectal carcinoma-derived epithelial cell line characterized by aggressive proliferative properties. It is commonly used in colorectal cancer therapy research, apoptosis studies, cell cycle analyses, and chemotherapy sensitivity evaluations.

SW620 – Human Metastatic Colon Cancer Cell Line

SW620 is an aggressive metastatic colorectal adenocarcinoma-derived cell line. Due to its metastatic characteristics, it is widely preferred in migration, invasion, and metastasis mechanism studies. It is considered a powerful model for antimetastatic agent evaluation.

HUH-7 – Human Liver Cancer Cell Line

HUH-7 is a human hepatocellular carcinoma-derived adherent epithelial cell line. It is extensively used in liver cancer research, hepatotoxicity analyses, antiviral studies, and evaluation of biologically active compounds on hepatic systems.

HeLa – Human Cervical Cancer Cell Line

HeLa is an immortal human cervical adenocarcinoma-derived cancer cell line. Due to its rapid proliferation rate and high experimental stability, it is widely used in general cytotoxicity screening, gene expression studies, viral oncology, and anticancer agent evaluations.

MCF-7 – Human Breast Cancer Cell Line

MCF-7 is a hormone receptor-positive human breast adenocarcinoma-derived epithelial cell line. It is commonly used in breast cancer biology studies, estrogen receptor-associated mechanisms, and anticancer agent research.

A549 – Human Lung Cancer Cell Line

A549 is a human alveolar lung adenocarcinoma-derived epithelial cell line. It is frequently used in lung cancer biology, nanoparticle toxicity studies, drug delivery system research, and respiratory system-oriented cytotoxicity analyses.

U-87 – Human Glioblastoma Cell Line

U-87 is an aggressive human glioblastoma-derived brain tumor cell line. It is widely utilized in brain tumor biology, proliferation mechanism studies, migration assays, and invasion analyses.

C6 – Rat Glioma Cell Line

C6 is a rat glioma-derived glial tumor cell line. It serves as an important experimental model in neuro-oncology, glioma biology, and anticancer agent research.

SH-SY5Y – Human Neuroblastoma Cell Line

SH-SY5Y is a human neuroblastoma-derived neuronal-like adherent cell line. It is widely used in neurodegenerative disease models, neurotoxicity studies, oxidative stress research, and nervous system-related cancer investigations.

K562 – Human Chronic Myeloid Leukemia Cell Line

K562 is a suspension cell line derived from human chronic myeloid leukemia. It is commonly used in leukemia biology, hematological malignancy studies, proliferation analyses, and anticancer drug evaluations.

DOHH2 – Human B-Cell Lymphoma Cell Line

DOHH2 is a human B-cell lymphoma-derived suspension cell line. It is a suitable model for lymphoma-oriented cytotoxicity analyses, immunological studies, and hematological cancer research.

FaDu – Human Pharyngeal/Laryngeal Cancer Cell Line

FaDu is a human pharyngeal/laryngeal squamous cell carcinoma-derived cell line. It is widely used in proliferation studies, migration assays, and radiotherapy sensitivity evaluations related to head and neck cancers.

PC3 – Human Prostate Cancer Cell Line

PC3 is an aggressive metastatic human prostate adenocarcinoma-derived cancer cell line. It is frequently utilized in advanced prostate cancer, metastasis, and antiproliferative agent studies.

HEALTHY / CONTROL CELL LINES

BEAS-2B – Human Bronchial Epithelial Cell Line

BEAS-2B is an immortalized non-cancerous human bronchial epithelial cell line. It is widely used as a control model in respiratory toxicity, nanoparticle safety, and comparative cytotoxicity studies.

HaCaT – Human Keratinocyte Cell Line

HaCaT is an immortalized healthy human epidermal keratinocyte cell line. It is extensively utilized in dermal toxicity, biocompatibility, cosmetic safety, and wound healing studies.

HS 738.St/Int – Human Gastric/Intestinal Stromal-Fibroblast Cell Line

HS 738.St/Int is a non-transformed healthy fibroblast-like cell line derived from human fetal gastric/intestinal stromal tissue. It is not a cancer cell line. This model is widely used in selective cytotoxicity, biocompatibility, and normal cell safety evaluations, particularly in gastrointestinal system-related studies.

L929 – Mouse Fibroblast Cell Line

L929 is a healthy mouse fibroblast-derived control cell line. It is widely used as a standard model in ISO biocompatibility testing, biomaterial safety analyses, and general cytotoxicity studies.

HUVEC – Human Umbilical Vein Endothelial Cell Line

HUVEC cells are healthy vascular endothelial cells derived from human umbilical veins. They are widely used in angiogenesis, vascular biology, biocompatibility, vascular toxicity, and wound healing studies.

NEUROLOGICAL / NEUROINFLAMMATORY MODELS

HT-22 – Mouse Hippocampal Neuronal Cell Line

HT-22 is a mouse hippocampal neuronal cell model. It is widely utilized in oxidative stress, glutamate toxicity, neurodegeneration, and neuroprotective agent studies.

BV-2 – Mouse Microglial Cell Line

BV-2 is a mouse microglial cell model commonly used in neuroinflammation, immune response, oxidative stress, and central nervous system inflammation studies.

Contact Information
For service inquiries and detailed information:

📧 info@synphrm.com